POLYPEPTIDE PREPARED FROM LEECH TISSUE OR SECRETION HIRUDINARIA MANILLENSIS SPECIFICALLY INHIBITING THROMBIN

摘要

FIELD: medicine. SUBSTANCE: polypeptide isolated from leech of species Hirudinaria manillensis has the following amino acid sequence: X-Y-Tyr-Thr-Asn-Cys-Thr-Gln-Ser-Gly-Gln-Asn-Tyr-Cys-Leu-Cys- Val-Gly-Ser-Asn-Val-Cys-Gly-Glu-Gly-Asp-Asn-Cys-Asn-D-Gln-Leu- Ser-Ser-Ser-Gly-Asn-Gln-Cys-Val-E-Gly-Gln---Gly-Thr-Pro-F-Pro- Gln-Gln-Ile-Pro-Asp-Gln-Z-Ile--Lys. In this sequence X, Y and Z are any amino acid residue; D Cys or Pro; E Gly or Asp or His; F Asp, Lys or Try, and also pharmaceutically acceptable salt, derivative or biological precursor on this base. Polypeptide and its fragments are specific antithrombins. EFFECT: leech polypeptide preparing. 5 cl, 6 dwg