Isothermal Strand Displacement Nucleic Acid Amplification (Isothermal Strand Displacement Nucleic Acid Amplification)

摘要

A method for amplifying a target nucleic acid sequence using a nucleic acid polymerase lacking 5 'exonuclease activity and a set of oligonucleotide primers. Preferably, a primer array is used. The primer array contains two sets of primers, one set containing two or more complementary primers. The other set contains two or more sense primers. Using the methods described, amplicons can be amplified under essentially constant environmental conditions without exonuclease activity or limited endonuclease activity.