A method for measuring the concentration of a immuno - reactants with the use of electrochemiluminescence

摘要

A method of measuring the concentration of an immunoreactant, such as antigen or antibody, in a liquid sample by use of an electrode on which is immobilized a complementary immunoreactant, such as antibody or antigen, capable of specifically reacting with the immunoreactant. An immunoreactant or complementary immunoreactant labeled with a chemiluminescent acridinium compound is bound to an electrode by an antibody-antigen reaction. By use of the electrode with the labeled immunoreactant bound thereon, electrochemical reduction of dissolved oxygen in an electrolytic solution is carried out to generate hydrogen peroxide on the electrode. The hydrogen peroxide triggers chemiluminescence, and the amount of luminescence is measured. According to the method, the amount of luminescence changes correspondingly to minute change in the concentration of the immunoreactant to be measured in the liquid sample and, therefore, it is possible to measure the concentration of the immunoreactant extremely accurately.