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摘要

The present invention discloses functional and selectable micro-Ti plasmids. The hygromycin phosphotransferase (aphIV) gene from Escherichia coli was inserted between the 5 min promoter and associated amino terminal region-encoding sequence of an octopine synthetase gene and the 3 min terminator signal sequence of anopaline synthetase gene. These constructs were assembled between T-DNA border fragments in a broad-host-range vector and used to create antibiotic-resistant plant cells.