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A process for the matrix - assisted laser desorption and ionization of analyte molecules, in particular of fragile or unstable molecules
A process for the matrix - assisted laser desorption and ionization of analyte molecules, in particular of fragile or unstable molecules
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机译:用于基质的方法-辅助激光解吸和电离分析物分子,特别是易碎或不稳定的分子
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摘要
Matrix-assisted laser desorption and ionisation (MALDI) of analyte molecules (I), especially fragile and/or labile molecules, is effected using a combination of two laser light-absorbing matrices. One matrix (M-1) is used for desorption of (I). When exposed to a pulsed laser, the other matrix (M-2) releases a gas, which expands adiabatically together with (I) in the vacuum used in the process. A pulsed laser with a wavelength above 200 nm is used.
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