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Production manner of the new chitosanase, and its chitosanase, and production manner null of the chitosan oligosaccharide which uses that
Production manner of the new chitosanase, and its chitosanase, and production manner null of the chitosan oligosaccharide which uses that
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机译:新壳聚糖酶及其壳聚糖酶的生产方式,以及使用该壳聚糖寡糖的生产方式无效
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摘要
PURPOSE:To prepare a novel chitosanase having excellent chitosan-decomposing ability, highly stable against metals, etc., and useful for preparing chitosan oligosaccharide by culturing a specific microorganism having an ability to produce the novel chitosanase and subsequently collecting the produced novel chitosanase. CONSTITUTION:A microorganism (e.g. Bacillus sp.PI-7S, FERM 9843) having an ability to produce the novel chitosanase is cultured in a medium and the produced novel chitosanase is collected. The chitosanase has substrate specificities having a good chitosan-decomposing ability and not having a carboxymethylcellulose-decomposing ability and further has the following characteristics: the optimal pH: 3.8-6.8; stable pH: 3.3-7.4; molecular weight measured by a SDS-polyacrylamide gel electrophoresis method: 25000+ or -3000; inhibiting substances: Ag+, Hg+ and p-chlorobenzoic acid mercuric salt and inhibiting substances are relatively few.
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