Recombinant proteinase from Clostridium histolyticum and its use for isolation of cells and cell populations

摘要

PCT No. PCT/EP95/05054 Sec. 371 Date Aug. 26, 1997 Sec. 102(e) Date Aug. 26, 1997 PCT Filed Dec. 20, 1995 PCT Pub. No. WO96/19583 PCT Pub. Date Jun. 27, 1996Process for disintegrating cell tissue and releasing cells or groups of cells contained therein by incubating the cell tissue with a recombinant neutral protease from Clostridium histolyticum which is coded by a) a DNA of nucleotides 1027-1965 from SEQ ID NO:3 or a DNA which is complementary thereto, b) nucleic acids which hybridize with a DNA of nucleotides 1027-1965 from SEQ ID NO:3, c) nucleic acids which, without the degeneracy of the genetic code, would hybridize with one of the nucleic acids mentioned in a) or b) and is the product of a prokaryotic or eukaryotic expression of an exogenous nucleic acid, until the cells or groups of cells have been released to the desired extent and separating the cells or groups of cells from the cell tissue fractions.