bankomaishin or risutosechin tolerance variant and its manufacturing method

摘要

PURPOSE:To produce a mutant resistant to inhibition of cell membrane synthesis in high sufficiency by subjecting a microorganism in Bacillus, producing an extracellular enzyme to mutagenic treatment and culturing the mutant in the medium containing a cell membrane synthesis inhibitor. CONSTITUTION:A microorganism in Bacillus, producing extracellular enzymes (parent strain) is treated with a mutagenic agent, irradiated with ultraviolet rays or radiation to cause mutation. Then, the mutant is cultured in a medium containing a high concentration of vancomycin or ristocetin to select the strain resistant to the inhibition of cell membrane synthesis. The parent strain is, e.g., Bacillus s.p. KSM-655, Bacillus sp. KSM-425. The cell membrane synthesis inhibitor is, e.g., 5-bromouracil or acridine or nitrous acid.