A closure structure for a vacuum specimen collection container is obtained which can enjoy high productivity and maintain a vacuum pressure within the specimen collection tube and which is excellent in needle cannula penetrating property and needle hole sealability. A closure structure 1 which can be detachably fitted in an open end of a specimen collection container in an airtight manner to maintain a vacuum condition within the specimen collection container, the closure structure including a grip section 2, a rubber elastic needle cannula penetrating portion 3 disposed to fill up a through-hole 2c in the grip section 2 and a rubber-like elastic fitting portion 4 configured to follow an interior surface profile at the open end of the specimen collection container to thereby fit therein in a airtight fashion, said grip section 2 having a higher rigidity relative to the needle cannula penetrating portion 3 and the fitting portion 4, and said tubular side wall 2a of the grip section 2 having on its inner side 2d at least one raised portion 2e or recessed portion for kickback preventive purpose. IMAGE
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