ASSAYS FOR MODULATORS OF THE INTERACTION BETWEEN ANAPHASE PROMOTING COMPLEX PROTEIN APC2 AND OTHER APC MEMBERS

摘要

The present invention relates to the finding of a novel component of the yeast anaphase promoting complex (APC) and its use in assays for inhibitors or modulators of cell cycle progression, particularly in fungal cells. In particular the invention provides an assay for an inhibitor of eukaryotic cell cycle progression which comprises (a) providing a polypeptide which comprises the sequence of the APC2 protein, or a fragment or variant thereof capable of associating with an Anaphase Promoting Complex (APC); (b) providing a second polypeptide member of the APC with which said APC2 protein is capable of forming a complex in the absence of a putative inhibitor; (c) providing a putative inhibitor compound; and (d) measuring the degree of inhibition of binding between (a) and (b) caused by the presence of said putative inhibitor. The second component is preferably APC8 (Cdc23).