Enzyme having LeuDH activity, nucleotide sequence coding therefor and process for the preparation of this enzyme

摘要

DNA sequence (I) from Bacillus cereus that encodes an enzyme (II) with leucine dehydrogenase activity (LeuDH) is: (a) an approximately 1.7 kb fragment (A), referred to in the specification but not reproduced; (b) a sequence equivalent to (a) within the degeneracy of the genetic code, and (c) a sequence that hybridises to (a) and/or (b). Also claimed are: (1) vectors containing (I) and capable of autonomous replication in Escherichia coli, and (2) E. coli with increased LeuDH productivity containing (1).