Functional screening and screening system of cervical cancer chemotherapy

摘要

The present invention relates to a method for producing a plasmid pRSV-luc comprising a RSV promoter gene inducing activity of human Papillomavirus (HPV) 16 type E6 protein and a reporter gene encoding a luciferase protein, To produce a SiHa / pRSV-luc cell line (KCTC 04227BP) and to provide a system for retrieving the survival performance of the HPV type 16 E6 protein. In addition, the present invention has an excellent effect that can be used for screening and screening for the efficacy of anticancer agents for cervical cancer by treating the expression of HPV 16 type E6 protein by treating an anticancer agent of cervical cancer with SiHa / pRSV-luc cell line.