Development of In situ Hybridization to detect Mycoplasma hyopneumoniae in formalin-fixed paraffin-embedded tissues without bacterial isolation

摘要

The invention organization as in on of porcine epidemic pneumonia diagnostic method using the hybridization method, and more particularly, porcine epidemic pneumonia (swine enzootic pneumonia, SEP) of Mycoplasma high ohnyu ammonia-specific DNA probes (probe of pathogens of ) for using the tissue hybridization method (in situ hybridization) to apply to the tissue section for a fixed pig in formalin relates to a rapid method for assessing the porcine epidemic pneumonia in diagnosing the infections, and the distribution state of the pathogen without bacteria . Accordingly, such in accordance with the present invention, the present invention uses the lung tissue fixed in formalin, because it was able to overcome the problems of the fluorescent antibody method, which use only the frozen tissues, and Mycoplasma high ohnyu ammonia only to detect the specific gene because we were able to overcome the problems of poorer immunohistochemical specificity due to cross-reactivity. And, observing not only solve even necessarily problems that require fresh tissue, indicating a high sensitivity as the polymerase gene level method using a chain reaction, the cells are infected with mycoplasma high ohnyu ammonia within the organization through an optical microscope It can be a quick and easily effect to diagnose infection with the distribution conditions of the pathogen.