Genes of heat-resistant DNA polymerase isolated from Thermos Filippomis and amino acid sequences inferred therefrom

摘要

The present invention relates to a heat resistant DNA polymerase gene isolated from Thermus filiformis and amino acid sequences inferred therefrom. The present inventors have spent mousse Filippo misses to genomic DNA, using a portion of the Taq pol gene as a probe from Ⅰ Tfi DNA polymerase, and screening of fragments containing the gene, cloned by colony hive electrical fragments in E. coli (T. filiformis) a hybridization screening the positive clones containing the Tfi DNA polymerase gene fragment as hybridization method, and then remove the Tfi DNA polymerase gene fragments from the clones found to be positive in that the electrical positive clone including Tfi DNA polymerase gene fragment Reconfirmed. Then, Tfi DNA polymerase by determining the base sequence of the gene fragment, and the deduced amino acid sequence from the electric base sequence, as a result of comparison with amino acid sequence of the conventional heat-resistant DNA polymerase and E. coli DNA polymerase, of the present invention Tfi DNA The polymerase was identified as a novel heat resistant DNA polymerase having high sequence homology with a conventional DNA polymerase. The Tfi DNA polymerase gene of the present invention may be useful for industrially mass-producing heat-resistant DNA polymerase or producing a heat-resistant DNA polymerase having improved function compared to wild-type enzyme through genetic engineering.