Production method of urease agent of Helicobacter pylori from recombinant E. coli

摘要

The present invention relates to a method for producing an antigenic determinant site protein of Helicobacter pylori (H. pylori), Urease A, in the form of a fusion protein using E. coli, and relates to a method for producing a urease A gene of Helicobacter pylori Ub-Ure A fusion protein expression vector containing a fusion gene in which histidine and ubiquitin gene are linked, is cultured under conditions suitable for expression of the fusion protein, and the recombinant E. coli cells expressing the fusion protein are treated with a guanidine salt Ub-Ure A fusion protein of the present invention comprising a step of subjecting the supernatant obtained by pulverization in an included buffer solution to histidine-binding affinity affinity chromatography of the present invention is used for the diagnosis of H. pylori infection and the development of a vaccine The His-Ub-Ure A fusion protein which can be usefully used can be mass-produced at a high yield.