method of obtaining dna vectors based on the bacteriophage lambda

摘要

method of obtaining dna vectors based on the bacteriophage lambda with the release of u0444u0430u0433u043eu043bu0438u0437u0430u0442u0430 from bacterial cells and their debris concentration. u0444u0430u0433u043eu0432 u043fu043eu043bu0438u044du0442u0438u043bu0435u043du0433u043bu0438u043au043eu043bu0435u043c deposition, centrifugation in caesium chloride density gradient purified dna and phenol - u0434u0435u0442u0435u0440u0433u0435u043du0442u043du044bu043c method, a fact that, with the c spruce productivity methodthe release of u0444u0430u0433u043eu043bu0438u0437u0430u0442u0430 from bacterial cells and their debris are u0441u0435u043fu0430u0440u0438u0440u043eu0432u0430u043du0438u0435u043c and an u0444u0430u0433u0430 implemented by u0438u043eu043du043eu043eu0431u043cu0435u043du043du043eu0439 chromatograph and on the column with u0434u044du0430u044d - u0446u0435u043bu043bu044eu043bu043eu0437u043eu0439, balanced 0.05 - 0.1 m potassium sodium u0444u043eu0441u0444u0430u0442u043du044bu043c buffer solution, the u044du043bu044eu0446u0438u044e are 0.4 and 1.0 m with the sodium.