Binding partner for inhibitors of cyclin dependent kinases and their use for the search for inhibitors, for the diagnosis or for the therapy of a disease

摘要

Independent claims are included for the following: (1) a protein that inhibits p27 and thereby arrests p27-induced inhibition of cell proliferation; (2) the protein of (1) comprising at least part of the amino acid sequence of p163 (I) Ämurine p163, a fully defined sequence of 466 amino acids given in the specificationÜ; (3) a protein that can be derived from the protein of (2) by deletion of the p27 binding domain or the Ran binding domain; (4) a protein that can be derived from the protein of (2) by deletion of all amino acid sequences other than the p27 binding domain; (5) a protein that is the human or other mammalian species homologue of a protein as in (1)-(4); (6) DNA coding for a protein as above; (7) antibodies and antibody fragments that bind to the p27 binding ÄdomainÜ of a protein as above; (8) antibodies and antibody fragments that bind to the Ran binding domain of a protein as above; (9) antisense nucleic acids complementary to portions of the DNA of (6) between codons 121 and 467; (10) a nucleic acid construct coding for an antisense nucleic acid as in (9) for inhibiting the proliferation of a cell, in which DNA coding for the antisense nucleic acid sequence is linked to at least one activation sequence and is introduced into the target cell as naked DNA or as an insert in a nonviral or viral vector; and (11) a nucleic acid construct containing the DNA of (6) linked to an activation sequence that permits expression of a protein as above in a cell.