New polygalacturonase prepared by expressing genes from Saccharomyces cerevisiae in micro-organisms, used to degrade pectin, e.g. for clarifying fruit juice

摘要

A new polygalacturonase (I) is prepared by expressing a 1083 bp gene (2, sequence reproduced), introduced by genetic engineering into the genome of a micro-organism. Independent claims are also included for the following: (a) a recombinant plasmid containing (2); (b) strains of yeast, particularly Saccharomyces cerevisiae that have (2) inserted into their genomes and are able to express (I); and (c) the recombinant production of (I) by culturing cells of (b) on a standard medium and recovering product from the supernatant.