Detection of single base mutations and other variations in double stranded DNA by conformation-sensitive gel electrophoresis

摘要

Simple methods of detecting single base pair mutations and other mutations in nucleic acid sequences are provided comprising generating a sample which may comprise heteroduplexes and homoduplexes and performing gel electrophoresis on the sample. The sample may be analyzed for one or more isolated high-melting domains prior to performance of gel electrophoresis. Kits are also provided.