PLANT-OPTIMIZED POLYNUCLEOTIDES ENCODING PESTICIDAL 43F- AND 80JJ1/130-TYPE PROTEINS

摘要

The subject invention concerns materials and methods useful in the control of pests and, particularly, plant pests. More specifically, the subject invention provides new, plant-optimized polynucleotide sequences that encode pesticidal toxins. The polynucleotide sequences of the subject invention have certain modifications, compared to wild-type sequences, that make them particularly well-suited for optimized expression in plants. Using the polynucleotide sequences described herein, the transformation of plants can be accomplished, using techniques known to those skilled in the art, in order to confer pest resistance upon said plants. The subject polynucleotide sequences can also be truncated to produce truncated toxins and to produce hybrid or chimeric (fusion) genes and proteins. In one preferred embodiment, the subject invention provides plant-optimized polynucleotide sequences which encode a full-length, approximately 130 kDa B.t. toxin protein that can be referred to as a 80JJ1/130-type protein. In another preferred embodiment, the subject invention provides plant-optimized polynucleotide sequences which encode toxin proteins that can be referred to as 43F-type toxins.