Rapid inducible beta-lactamase screen test

摘要

In an assay for determination of beta-lactam antibiotic resistance in a target bacterial strain, the strain is grown in the presence of both a beta-lactamese inducing antibiotic and a beta-lactam indicator antibiotic [which kills or inhibits the growth of bacteria] unable to hydrolyze beta- lactam antibiotics. Growth, indicative of drug resistance in the target strain, is monitored by detecting a fluorophor released by the enzymatic cleavage of a metabolizable fluorogenic compound.