The procedure of cultivation for massive mamu00ecfero cells to obtain recombinant human erythropoietin, and recombinant human erythropoietin obtained with this procedure

摘要

b procedure of cultivation for massive mamu00ecfero cells to obtain recombinant human erythropoietin and recombinant human erythropoietin obtained with this procedure d .The present invention patent describes a process for mass cultivation of mammalian cells producing recombinant EPO.The production process follows the different stages of expansion, starting from viable cells stored frozen.Subsequently, is a stage production in which is used the means of cultivation, specially formulated to minimize the aggregate of protu00eaicos supplements.Supplementation with insulin because of unexpectedly high productivity of EPO with high purity in the culture medium collected. This is one of the key aspects of the method used.The supernatant of cultivation is finally concentrated to obtain a greater concentration of EPO in a form suitable to be used as obtained or purified subsequently for the uses that AssiM the need.