Production of recombinant monelina using the expression system in yeast metilotru00f3ficas

摘要

Invention patent: b "production of recombinant monelina using expression system in yeast metilotru00f3ficas" d .The present invention relates to a protein similar to monelina of single chain that is stable and that it is at least 100 times sweeter when compared with sucrose based on weight.The present invention also relates to a nucleic acid encoding said protein similar to the monelina.Preferably,The nucleic acid comprises a promoter and a sequ00b3u00eancia signal to the direction of the expression and secretion of the protein similar to the monelina encoded in the yeast Pichia metilotru00f3fica pastOris.The present invention also relates to a recombinant Pichia pastoris cell which contains a nucleic acid encoding protein similar to the monelina,A process for obtaining protein similar to the monelina of Pichia pastoris recombinant and the product of the process.