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DESIGN OF A POLYEPITOPIC CONSTRUCT FOR THE INDUCTION OF HLA-A2.1 RESTRICTED HIV 1 SPECIFIC CTL RESPONSES USING HHD MICE
DESIGN OF A POLYEPITOPIC CONSTRUCT FOR THE INDUCTION OF HLA-A2.1 RESTRICTED HIV 1 SPECIFIC CTL RESPONSES USING HHD MICE
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机译:使用HHD小鼠诱导HLA-A2.1限制性HIV 1特异性CTL反应的多表位构建体的设计
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摘要
H-2 class I negative, HLA-A2.1 transgenic HHD mice were used for a comparative evaluation of the immunogenicity of HLA-A2.1 restricted human tumour-associated CTL epitopes and HIV 1-derived epitopes. A hierarchy was established among these epitopic peptides injected into mice in IFA which correlates globally with their capacity to bind and stabilize HLA-A2.1 molecules. A tyrosine substitution in position 1 of the HIV 1-derived epitopic peptides, which increases both their affinity for and their HLA-A2.1 molecule stabilizing capacity, was introduced in a significant proportion of them. DNA immunizations were performed using a construct comprising nucleic acids encoding the epitopes inserted into the pre-S2 segment of the hepatitis B middle glycoprotein. CTL responses against most of the inserted epitopes could be elicited simultaneously in a single animal.
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机译:使用H-2 I类阴性,HLA-A2.1转基因HHD小鼠对HLA-A2.1限制性人类肿瘤相关CTL表位和HIV 1衍生表位的免疫原性进行比较评估。在注射到IFA小鼠中的这些表位肽之间建立了一个层次,该层次与它们结合和稳定HLA-A2.1分子的能力总体相关。 HIV 1衍生的表位肽的位置1的酪氨酸取代,增加了它们对HLA-A2.1分子的亲和力和稳定能力,在其中有很大一部分被引入。使用包含编码插入到乙型肝炎中间糖蛋白的前S2节段中的表位的核酸的构建体进行DNA免疫。可以在一只动物中同时引发针对大多数插入表位的CTL反应。
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