首页>
外国专利>
Agrobacterium tumefaciens transformation of musa species
Agrobacterium tumefaciens transformation of musa species
展开▼
机译:农杆菌属根癌农杆菌转化
展开▼
页面导航
摘要
著录项
相似文献
摘要
A method for transforming a Musa plant comprises:a. wounding meristematic tissue from a Musa plant by microparticle bombardment to generate a wounded Musa plant tissue and to facilitate access of Agrobacterium tumefaciens to Musa plant cells competent for transformation and regeneration;b. applying to said wounded Musa plant tissue at least one transformation competent Agrobacterium tumefaciens to transform said Musa plant, wherein said at least one transformation competent Agrobacterium tumefaciens harbours at least one Ti plasmid and at least one virulence gene, wherein said at least one Ti plasmid comprises at least one genetically engineered T-DNA to effect transformation of said Musa plant;c. growing said transformed Musa plant for a sufficient time to identify the presence of chimeric features;d. producing nonchimeric tissue by dividing said transformed Musa plant into segments which have at least one meristem which can regenerate into an intact plant and which have cells that are uniformly transformed to produce nonchimeric tissue; ande. growing said nonchimeric tissue into a nonchimeric plant.展开▼
机译:一种穆萨 I>植物的转化方法,包括: a。用微粒轰击法将 Musa I>植物的分生组织弄伤,产生受伤的 Musa I>植物组织,并促进根癌农杆菌 I>进入 Musa I>具有转化和再生能力的植物细胞; ListItem> b。向所述受伤的 Musa I>植物组织施加至少一种转化能力的根癌农杆菌 I>以转化所述 Musa I>植物,其中所述至少一种转化能力的农杆菌根癌农杆菌具有至少一种Ti质粒和至少一种毒力基因,其中所述至少一种Ti质粒包含至少一种基因工程改造的T-DNA以实现所述 Musa I>植物的转化; ListItem> c。使所述转化的 Musa I>植物生长足够长的时间,以识别嵌合特征的存在; ListItem> d。通过将所述转化的 Musa I>植物分成具有至少一个分生组织的片段来产生非嵌合组织,所述分生组织可以再生成完整的植物,并且所述细胞具有被均匀转化以产生非嵌合组织的细胞;和 ListItem> e。将所述非嵌合组织生长为非嵌合植物。 ListItem> UnorderedList>
展开▼