Method and a kit for the determination of dna - double - / splits off and apparatus for controlled filtering of in particular dna - molecules and / or other molecules / molecular complexes containing liquid media with well - filter plates

摘要

It is, inter alia, a method for the determination of dna - double-strand breaks, respectively, is provided with the following steps: dollars a a.) It is an object of cells to be investigated and / or fabrics on specific filter; dollars a b.) Lysis of the cells / fabric with at least one detergent, at least one chaotropic substance and at least one specific dna - double strand binding fluorescent dye; dollars a is c.) Elution of the through the lysis freigesezten dna - fragments by the specific filter; dollars a d.) An evaluation is carried out by fluorescence spectroscopy, dollar a or dollars a a.) It is an object of cells to be investigated and / or fabrics on specific filter; dollars a b.) Lysis of the cells / fabric with at least one detergent, at least one chaotropic substance; dollars a is c.) Elution of the released by the lysis dna - fragments by the specific filter; dollars a d.) The shift of the eluates obtained with at least one specific dna - double strand binding fluorescent dye; dollars a e.) An evaluation is carried out by fluorescence spectroscopy.