Macrophage cell line for antinuclear antibodies test; a method for the detection of said antibody by the use of this cell line, and preparation of lamellae

摘要

PCT No. PCT/KR93/00061 Sec. 371 Date Mar. 17, 1994 Sec. 102(e) Date Mar. 17, 1994 PCT Filed Jul. 21, 1993 PCT Pub. No. WO94/02594 PCT Pub. Date Feb. 3, 1994The macrophage cell line, IT-1 (KCCM-10038) was derived from normal human bone marrow. IT-1 cells are characterized by excellent adherence onto glass slides used in immunofluorescence assays, and the cells provide a positive result in the antinuclear antibody (ANA) test. Moreover, ANA tests performed with IT-1 cells were found to be more reliable and more easily interpreted than ANA tests performed with the customary HEp-2 cell line. Accordingly, IT-1 cells are particularly suitable for the diagnosis of autoimmune disease using the ANA test.