Method for detecting activity of acetyl transferase and deacetylation enzyme and screening method of inhibitor or promoter of these enzymes

摘要

Acetylation of substrate peptide by acetyl transferase and deacetylation of acetylated substrate peptide by deacetylase using antiacetylated peptide antibody, substrate peptit after these reactions We found that acetyl transferase activity and deacetylation enzyme activity were easily detected. In addition, an inhibitor or an inhibitor of acetyl transferase and deacetylase is conveniently used as an inhibitor of acetyltransferase and deacetylase It was found that it was possible to carry out the lining. We have also developed a screening system for inhibitors of acetylation or acetyltransferase.