Recombinant bHLH-PAS / JHR polypeptide, and its use for screening a part of a potential pesticide

摘要

Analogs of insect hormone (57), such as [summary] juvenile hormone, and I will be disclosed consists of antagonists of the hormone, for a promising group of insecticides environmentally safe. time consuming, bioassay conventional for juvenile hormone analogs, and screening for those that are potential antagonists that are inefficient cost applied. Recombinant bHLH-PAS juvenile hormone receptor isolated from methoprene resistance locus of Drosophila, binding assays in vitro and in vivo, can be used to discover insecticides targeting juvenile hormone form new I will provide the basis for. In addition, the nucleotide sequence of the bHLH-PAS / JHR polypeptide of Drosophila, a tool for the isolation of juvenile hormone receptor gene from another species of insect is provided.