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Screening of high production of libraries of DNA expressed in filamentous fungi

机译:高产丝状真菌中表达的DNA文库的筛选

摘要

"Screening of high production of libraries of DNA expressed in filamentous fungi. This invention provides a method for the expression of exogenous DNA libraries in filamentous fungi.The fungi are capable of processing eukaryotic genes contain introns, and may also result in post-translational processing steps, such as glycosylation and folding of proteins.The invention provides the use of fungi with altered morphology, which allows the screening of high production and molecular evolution oriented protein expressed.The same fungus transformed can be used (as illustrated in Figure 1) to produce larger quantities of protein for isolation, characterization, and testing applications.And can be suitable for the industrial production of the protein, too.
机译:高产丝状真菌中表达的DNA文库的筛选。本发明提供了一种在丝状真菌中表达外源DNA文库的方法。该真菌能够加工含有内含子的真核基因,还可能导致翻译后翻译加工步骤,例如糖基化和蛋白质折叠。本发明提供了具有改变的形态的真菌的使用,其允许筛选表达的高产量和分子进化导向的蛋白质。可以使用转化的相同真菌(如图1所示)。可以生产大量蛋白质以用于分离,表征和测试应用,也可以适用于蛋白质的工业化生产。

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