Identifisering av nye splicevarianter av den humane katalytiske subenheten C fra den cAMP-avhengige proteinkinasen oganvendelse derav

摘要

The present invention demonstrates that the Cβgene encodes at least 6 different gene products, designated Cβ1, Cβ2, Cβ3, Cβ4, Cβ4ab and Cβ4abc. As is the case with the murine and bovine splice variants, all the human Cβ splice variants vary in the N-terminal part preceding the part encoded by exon 2. Homologues to all Cβ splice variants identified in mouse and bovine were identified in human (Cβ1, Cß β2, Cβ3, and Cβ4) in addition to two novel Cβ splice variants (Cβ4ab and Cβ4abc), that have previously not been identified in any other species. The present invention includes in this respect genomic DNA- and cDNA sequences encoding said splice variants and comprises the nucleotide sequences shown in SEQ ID NO: 1, 2, 3, 4, 5 and 6 respectively. Wherein the said proteins are new splice variants of the Cβ protein. The present invention is further directed to vectors comprising said cDNA sequences. The invention also includes proteins characterised by the specific amino acid Cß β splice variant proteins shown in SEQ ID NO: 7, 8 and 9 respectively. The invention includes further use of the said Cβ splice variant proteins and DNA sequences in preparation of pharmaceuticals for diagnostic- and therapeutic purposes.