Kinetic turbidimetric method for measuring endotoxin or (1-3) -beta-D-glucan.

摘要

A process for measuring the amount of endotoxin or (1 - 3) {BE} -D-glucan (KNOWN FROM NOW ON {BE} glucan) in a sample, which involves mixing the sample with amebocyte lysate OF HORSESHOE CRABS IN THE PRESENCE OF AT LEAST A water-soluble polymer selected from the group consisting of a polyethylene glycol, polyvinyl alcohol, methyl cellulose and hydroxypropyl cellulose, apply a light to the resulting mixture, measure the time required until a degree of VARIATION optics resulting mixture reaches a value DEFAULT after mixing the sample with the amebocyte lysate or after having spent a period of time DEFAULT FROM MIX SIGN WITH amebocyte lysate, and determining the amount of endotoxin or DE gLUCAN} {BE PRESENT IN THE SAMPLE BASED ON THE RELATIONSHIP BETWEEN SAID TIME AND QUANTITY OF endotoxin and glucan {BE}; And a reagent for measuring the amount of endotoxin O {BE} GLUCAN present in a sample containing amebocyte lysate HORSESHOE CRABS AND at least one soluble polymer selected from the group consisting of a polyethylene glycol, polyvinyl alcohol, A methyl cellulose and hydroxypropyl cellulose.