A method for characterizing nucleic acid molecules which involves generating DNA fragments upstream and extensible resulting from the cleavage of a nucleic acid in an abasic site.

摘要

A method for characterizing nucleic acid molecules, comprising the steps of: i) introducing a modified base which is a substrate of a DNA glycosylase into a DNA molecule; II) excising the modified base by means of said DNA glycosylase so that an abasic site is generated; III) cleaving the DNA at the abasic site so that a DNA fragment upstream which can be extended is generated; and IV) incubating the extendible upstream fragment and in the presence of an enzyme that allows the extension and a template nucleic acid and analyzing the resultant fragment (s).