Galphasubq/subproetin variants and their use in the analysis and discovery of agonists and antagonists of chemosensory receptors

摘要

The invention provides a series of GalphaSUBq /SUBprotein variants that functionally couple to sensory cell receptors such as taste GPCRs (TRs) and olfactory GPCRs (ORs) in an overly promiscuous manner. According to the invention, the functional coupling can be determined, for example, by measuring changes in intracellular IP3, or calcium. In a particular embodiment, the GalphaSUBq /SUBprotein variants can be expressed in mammalian cell lines or Xenopus oocytes, and then evaluated using calcium fluorescence imaging and electrophysiological recording.