Maintenance of genic male-sterile female parental lines using a maintainer line with a stable engineered chromosome

摘要

The present invention provides a method for stably maintaining a genic malesterile female parental line of bread and durum wheat for the production of hybrid wheat. It also provides a male-sterile female line homozygous for a recessive malesterility allele and for a dominant pollen-killer allele, and a maintainer line that is readily and stably propagated. The maintainer line isogenic to the female line but has a stable alien engineered chromosomal arm, added to the wheat complement, carrying a dominant male-fertilizy allele that restores fertility to the maintainer line, a rece ssive pollen-killer allele which is sensitive to the dominant polen-killer allele thus causing abortion of pollen grains carrying it and thus preventing the transmission of this chromosome arm to the femal line, and a heterologous selectable marker that facilitates the selection of maintainer plants among the progeny of the selfed maintainer line and thus, maintenance of the maintainer itself.