METHODS AND MATERIALS TO DETERMINE THE P53 STATUS OF A SAMPLE BY DETERMINING THE BINDING OF P53 TO A VECTOR

摘要

The invention provides: (1) a method of determining the p53 status of a sample, the method comprising providing a sample containing a pGL3 vector and determining whether p53 binds to the pGL3 vector; (2) a method of modifying a pGL3 vector, the method comprising deleting or altering a CCCGGG motif of the pGL3 vector and/or deleting or altering a sequence within a 20 bp sequence 5' or 3' of said CCCGGG motif; (3) a modified pGL3 vector having an alteration or deletion in such a site; and (4) the use of nucleic acid having a sequence incorporating the CCCGGG motif to confer promoter activity.