Ornithine carbamoyl transferase gene and its use as a marker gene in a host-vector system for the production of proteins in basidiomycetes

摘要

This invention relates to a novel ornithine carbamoyl transferase (OCTase) gene, a recombinant DNA containing the OCTase DNA, a transformation system for a basidiomycete, and production of useful proteins using the system.;More particularly, this invention relates to the OCTase gene of Coriolushirsutus to afford an efficient host-vector system in basidiomycetes (particularly a white rot fungus such as Coriolushirsutus IFO 4917) for the preparation of useful proteins. OCTase is the enzyme to transform ornithine to citrulline in arginine biosynthesis in organisms. The present invention provides the OCTase genes of C.hirsutus, the useful Arg⁻ auxotrophic mutant of C. hirsutus deficient in the OCTase gene, the efficient condition for the preparation of protoplasts and the transformation of the mutant with the cloned OCTase gene and the recombinant DNA's including a promoter, a signal peptide-coding DNA and a protein-coding DNA, the construction of the novel host-vector system of C.hirsutus, and the new method of a large scale preparation of useful proteins using this novel host-vector system with a new recombinant DNA technique.;Furthermore, this invention provides a highly efficient method to produce a useful protein such as lignin peroxidase which is difficult to produce by the conventional method.