MONOCLONAL ANTIBODIES AGAINST LIPID STRUCTURES DIFFERENT FROM THE DOUBLE LAYER OF CELL MEMBRANES FOR DEFINING PHYSIOLOGICAL STATES IN DIFFERENT CELL TYPES AND DETECTING ANTI LIPID PARTICLE ANTIBODIES IN HUMAN BEINGS AND/OR ANIMALS SUFFERING FROM CERTAIN ANTIPHOSPHOLIPID ANTIBODY-RELATED DISEASES

摘要

The invention relates to the obtention of murine hybridomas that produce monoclonal antibodies against non double layer lipid particles also known as lipid particles in liposomes. The monoclonal antibodies are isotype IgM. Said antibodies were obtained by fusing spleen cells of female BALB/c mice that were immunized with liposomes of unilamellar phosphatidylcholine: phosphatidate (2:1) containing lipid particles induced by Mn+2 and P3X63AGBU.1 myeloma cell line. In the present invention, the use of said antibodies has been tested in ELISA assays to detect non dual layer lipid associations both in animal and plant cells. Hence its potential use in the prevention and treatment of diseases associated with the production of antiphospholipid antibodies.