METHOD FOR GENERATING CHROMOSOME REGION-SPECIFIC PROBES

摘要

the present invention relates to rapid and reproducible procedure for the production of a chromosome region specific probes (crsp) for diagnosis and research.these probes are produced by polymerase chain reaction (pcr) and direct in vitro chromosome dna and hybridized microdissu00e9quu00e9 from the chromosomal region to explore, this is followed by a marking in order to induce metaphase chromosomes and nuclei interpha hybridization i.we can further improve the specificity of the crsp by using line segment and a strategy of hybrid dna sequences bound adnl), prepared from dna libraries in chromosomal dna in situ, the microdissu00e9quer the chromosomal region to explore, and the amplified directly using the line segment as a primer.these procedures make it possible to produce a large number of probes specific for chromosome region without manipulation of microchemical after dissection and provide identification of cryptic chromosomal abnormalities that previously could not be detected by anal routine cytogenetic yses.we can also use the probes produced by the methods described for the screening of a dna library of interest.