METHOD OF SELECTING PLANT PROMOTERS TO CONTROL TRANSGENE EXPRESSION

摘要

The present invention relates to a method for the identification and cloning of promoters that are useful in regulation of gene expression under different environmental conditions, such as in cultured transformed cells or in transgenic plants. A promoter that is a nucleic acid region located upstream of the 5' end of a plant DNA structural coding sequence that is transcribed at desired and/or modulated levels in plant tissues. The promoter regions are capable of conferring high levels of transcription in leaf tissue and in developing seed tissues when used as a promoter for a heterologous coding sequence in a chimeric gene. The promoter and any chimeric gene in which it may be used can be used to obtain transformed plant cells and plants. Chimeric genes including the isolated promoter region, transformed plants containing the isolated promoter region, transformed plant cells and seeds are also disclosed.