IMPROVED CULTURE VESSEL HAVING NON-FLAT SURFACES FOR GROWING OR CULTURING CELLS, CELLULAR AGGREGATES, TISSUES AND ORGANOIDS AND METHODS FOR USING SAME

摘要

An improved culture vessel or bioreactor for growing cells, cellular aggregates or tissues includes a housing having at least one chamber defined by a curved wall symmetrical about an axis, an inlet and an outlet. The vessel has at least one filter in fluid communication with at least one of the inlet or outlet is structured to pass culture media and cells, cellular aggregates or tissues. The vessel housing is rotated about an axis to suspend the cells, preferably in non-linear toroidal flow. In a preferred embodiment, at least a portion of the chamber may have a substantially elliptoid, oblate elliptoid, spherical or oblate spherical shape and an unobstructed volume for growth of cells, cellular aggregates or tissues.