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CHEMILUMINESCENT DIALKYL-SUBSTITUTED 1,2-DIOXETANE COMPOUNDS, METHODS OF SYNTHESIS AND USE
CHEMILUMINESCENT DIALKYL-SUBSTITUTED 1,2-DIOXETANE COMPOUNDS, METHODS OF SYNTHESIS AND USE
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机译:化学发光的二烷基取代的1,2-二氧杂环丁烷化合物,合成方法和用途
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摘要
A chemiluminescent assay method and compositions are described which use a dialkyl-substituted dioxetane which is deprotected to trigger a chemiluminescent reaction. Chemiluminescent 1,2-dioxetane compounds substituted on the dioxetane ring with two nonspirofused alkyl groups which can be triggered by a reagent to generate light are disclosed. Dialkyl-substituted dioxetanes are useful for the detection of triggering agents including enzymes. The enzyme may be present alone or linked to a member of a specific binding pair in an immunoassay, DNA probe assay or other assay where the enzyme is bound to a reporter molecule.
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