CRYSTAL STRUCTURE OF PEROXIREDOXIN 5 AND ITS USE FOR DESIGN OF STRUCTURAL HOMOLOGUES

摘要

The present invention relates to a crystal comprising a PRDX5 protein in crystalline form, wherein said PRDX5 protein in crystalline form has the tetragonal space group symmetry P41 21 2.The invention further relates to a method for synthesizing a homologue of said crystal or an active binding site pocket , wherein said method comprises(a) providing the coordinates of the active binding site pocket of PRDX5, defined by residues of Table 2 and structure coordinates in Table 1, to a computer modeling system,(b) designing a homologue of said crystal or said active binding site pocket;(c) synthesizing said homologue,(d) optionally screening said homologue in a peroxidase activity assay for determining its catalytic activity, and(e) if desired, determining whether said compound inhibits oxidation in an antioxidant activity assay.