DETECTION OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA)

摘要

Described is a method for the detection and quantification of methicillin-resistant Staphylococcus aureus from a specimen comprising a) contacting the specimen with Anti-Protein A antibodies so as to adsorb methicillinresistant Staphylococcus aureus and/or methicillin-sensitive Staphylococcus aureus, b) separating said antibodies to which methicillin-resistant Staphylococcus aureus and/or methicillin-sensitive Staphylococcus aureus have been adsorbed from the specimen, c) lysing methicillin-resistant Staphylococcus aureus and/or methicillin-sensitive Staphylococcus aureus adsorbed to said antibodies so as to release their DNA, d) combining the released DNA with (i) probes and/or primers which are specific for a target DNA sequence of the mecA gene of methicillin-resistant Staphylococcus aureus and/or of methicillin-resistant Staphylococcus epidermis, (ii) probes and/or primers which are specific for a target DNA sequence of methicillin-resistant Staphylococcus aureus other than a target DNA sequence of the mecA gene and (iii) probes and/or primers which are specific for a target DNA sequence of methicillin-resistant Staphylococcus epidermis other than a target DNA sequence of the mecA gene, whereby the target DNA sequences of methicillin-resistant Staphylococcus aureus and of methicillin-resistant Staphylococcus epidermis are not homologous, e) subjecting the combined released DNA and the specific probes and/or primers to conditions which permit amplification of said target DNA sequences, f) detecting the presence and amount of the amplified target DNA sequences as an indication of the presence and amount of methicillin-resistant Staphylococcus aureus, new primers and probes and diagnostic kits for the detection and quantification of MRSA.