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A METHOD FOR SELECTIVE ELECTROFUSION OF AT LEAST TWO FUSION PARTNERS HAVING CELL-LIKE MEMBRANES

机译:具有细胞样膜的至少两种融合伙伴的选择性电融合方法

摘要

Disclosed is a method for selective electrofusion of at least two fusion partners having cell-like membranes and cellular or subcellular dimensions, comprising the following steps: A) the fusion partners are brought into contact with each other and B) an electrical field of a strength sufficient to obtain fusion and highly focused on the fusion partners is applied. The fusion partners are independently selected from the group consisting of a single cell, a liposome, a proteoliposome, a synthetic vesicle, an egg cell, an enucleated egg cell, a sperm cell at any development stage and a plant protoplast.
机译:公开了一种用于选择性电融合至少两个具有细胞样膜和细胞或亚细胞尺寸的融合伴侣的方法,其包括以下步骤:A)使融合伴侣彼此接触,以及B)具有一定强度的电场足以获得融合并高度关注融合伙伴的应用。融合伴侣独立地选自单细胞,脂质体,蛋白脂质体,合成囊泡,卵细胞,去核卵细胞,处于任何发育阶段的精子细胞和植物原生质体。

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