Fusion protein comprising integrase (phiC31) and a signal peptide (NLS)

摘要

The present invention concerns a fusion protein consisting of the site-specific DNA recombinase C31-Int of phage ΦC31 and a peptide sequence which directs the nuclear uptake of the fusion protein in eucaryotic cells, and the use of this fusion protein to recombine DNA molecules containing C31-Int recognition sequences in eucaryotic cells at high efficiency. In addition the invention relates to a cell, preferably a mammalian cell which contains said C31-Int recognition sequences in its genome and which are recombined by the said C31-Int fusion protein. Moreover, the invention relates to the use of said cell to study the function of genes and for the creation of transgenic organisms to study gene function at various developmental stages, including the adult. In conclusion, the present invention provides a process which enables the highly efficient modification of the genome of mammalian cells by site-specific recombination.