-12 A novel cell line PNUH-12 derived from a human squamous carcinoma in human hypopharynx and process for characterization

摘要

The present invention is a cell line derived from squamous cell carcinoma of the hypopharynx person (PNUH-12; accession No. KCLRF-BP-00022) as a method for establishing, the method comprising harvesting the malignant solid tumor tissue from patients with head and neck cancer; Implantation step by the tissue to sterile gentamicin (gentamycine) and fern Supreme (fungizone) in MEM medium containing the transfer to the cell growth chamber; After crushing the tissue under aseptic environment with 1 to 3㎜ size, processing overnight under proper conditions in collagenase and DNase B; The step of moving said tissue pieces with 10% fetal bovine serum, gentamicin (50㎍ / ㎖), you statin (50㎍ / ㎖) Petri dishes containing the MEM medium containing the observed while performing the culture; When the growth of epithelial cells and fibroblasts proceeds differential trypsinization and; Step of the process after the selective separation by scraping to remove the fibroblasts and the growth only pure tumor cell; Cells comprising more than 50 passages; And a step of establishing a more than 50 passages were squamous cell carcinoma cell line cultures and established properties.