a protease which degrades keratin layers of skin surface a microorganism which produces the protease and a cosmetic composition containing a culture solution thereof.

摘要

The present invention relates to a cosmetic composition containing the microorganism and the culture medium containing the protease producing this protease, the decomposition of the dead cell layer keratin component of the human epidermis cells derived.; The present inventors have in to separate the microorganisms to search for strains that produce enzymes that efficiently decompose skin impurities keratin for use in the composition of cosmetic products to produce other new protease from what is known so far from the soil Bacillus (Bacillus sp. HB-5, was identified by accession No. KCTC8959P), and review the properties of the enzyme and the nature of the strain, it has been found to the existing production strains and other characteristics. Strain of the present invention has a single subunit of a type of molecular weight 60,000 daltons (Da) to produce the protease, the reaction optimum pH is close to 11, the optimal temperature is in the range 50 ℃, at pH 8-12, 30 it is stable for up to -60 ℃. A production medium may be glucose as carbon source, a village school, fructose, starch, yeast extract as nitrogen, protein, phosphate, and magnesium salts such as human hair. Enzyme of the present invention is a serine protease, the loss of enzyme activity by the enzyme inhibitors such as PMSF, a novel enzyme stable, with a keratin substrate specificity by the high molecular weight compound such as glycerin, 1,3-butylene glycol, decomposing casein, gelatin, starch and the like, and by the addition or the like cosmetics, detergents may be used to remove keratin ingredient.