- -glucosidase from extreme thermophile Thermus caldophilus GK24 and its production

摘要

The invention thermophilic bacterium Summers knife filler's support K of a novel thermostable enzyme alpha isolated from the 24 strains Expression comprising a-glucosidase, a gene coding for the enzyme, the gene vector, transformed by the vector the complex sugar production process according to the starch hydrolysis and transfer reactions each using a glucosidase-glucosidase production method, the method comprising the alpha produced by the-recombinant strain, and the alpha, comprising culturing the recombinant strain It relates. Recombinant alpha according to the present invention - glucosidase Let the optimum temperature activity is 90 ℃, and optimum pH 6.5 iso maltooligosaccharides or useful in the sugar hydrolysis and dangjeon the catalyst to the reaction the starch industry, the food industry and biomedicine.