Recombinant Marek's disease virus type 2 expressing the S1 glycoprotein of infectious bronchitis virus and a method for constructing thereof

摘要

PURPOSE: A recombinant Marek's disease virus type 2(MDV2) expressing the S1 glycoprotein of infectious bronchitis virus(IBV) and its construction methods are provided, to effectively use in preventing the infectious bronchitis of chicken. CONSTITUTION: The Marek's disease virus type 2(MDV2) expression vector pKGFKM contains the S1 glycoprotein gene of infectious bronchitis virus(IBV). The recombinant Marek's disease virus type 2(MDV2) expressing the S1 glycoprotein of infectious bronchitis virus(IBV) rMDV2-US3S1 (KFCC 11154) is prepared by transforming the expression vector pKGFKM. The recombinant Marek's disease virus type 2(MDV2) expressing the S1 glycoprotein of infectious bronchitis virus(IBV) is constructed by co-transfecting Marek's disease virus type 2(MDV2) expression vector pKGFKM containing the S1 glycoprotein gene of infectious bronchitis virus(IBV) and genomic MDV HPRS24 DNA into the CEF cell by using electroporation method.